Products Catalog - 1

Protein Expression Systems Vectors and reagent kits for high-fidelity, cell-free, human in vitro translation (human protein expression) and luciferase assays based on spectrally distinct Cypridina, Gaussia, Renilla and firefly luciferase reporters.  

• In Vitro Protein Expression Systems Vectors, plasmid controls and cell extracts for high-fidelity, cell-free in vitro translation, recombinant protein expression and post-translational modification from RNA or DNA templates.  
• Luciferase Reporter Assays Luciferase reporter reagents, multiple cloning site vectors, and flash, glow and dual luciferase assay kits based on spectrally resolvable Cypridina, Gaussia, Renilla and firefly luciferases.  

Cell Lysis and Protein Extraction Protease inhibitors, phosphatase inhibitors, detergents and specialized reagents designed for efficient organelle isolation, subcellular fractionation and protein solubilization from a variety of cell and tissue types. Tissue Strainer Rapidly separate cells or cell lysate from tissue debris after mechanical disruption using a 250-micron mesh filter and gravity or gentle centrifugation.  

• Pierce Cell Lysis Reagents for Protein Extraction Gentle whole cell lysis buffers for high-yield protein extraction and solubilization from E. coli and other bacteria, cultured mammalian cells and tissues, insect cells, yeast cultures, and flowering plants.  
• Organelle Isolation and Enrichment Kits Optimized reagent kits for subcellular fractionation of proteins and isolation of organelles such as nuclei, mitochondria, peroxisomes and lysosomes by Dounce-homogenization or density-gradient separation.  
• Subcellular Fractionation Kits Reagent-based kits optimized for stepwise separation, enrichment and extraction of proteins from different cell fractions; isolate cytoplasmic, membrane, nuclear, chromatin-bound and cytoskeletal proteins for downstream analysis.  
• Protease Inhibitors and Phosphatase Inhibitors Ready-to-use, inhibitor cocktail solutions and tablets, as well as individual purified formulations of protease inhibitors and phosphatase inhibitors to protect proteins from degradation during cell lysis and extraction.  
• Enzymes for Cell Lysis Digesting enzymes such as lysozyme, deoxyribonuclease I (DNase I), Universal Nuclease for Cell Lysis and micrococcal nuclease (MNase) to aid in protein extraction, subcellular fractionation, organelle isolation and chromatin IP.  
• Stabilizing, Solubilizing and Refolding Reagents Supporting reagents and kits to stabilize functional enzymes and proteins for storage, solubilize inclusion bodies for recombinant protein purification, and optimize buffer conditions for refolding denatured protein structures.  
• Detergents for Cell Lysis High-purity, low-peroxide, surfactant solutions and detergent solids for use in cell lysis reagent formulation, protein solubilization procedures, wash buffers for ELISA, and other protein research methods. 
• Detergent Removal Resins and Kits Affinity columns and precipitation reagents to remove detergents and surfactant micelles from protein extracts and other processed biological samples; eliminate detergent interference to improved compatibility with downstream applications. 

Protein Affinity Purification Agarose beads and other resins for specific purification of proteins and other target molecules, kits for immunoprecipitation (IP), activated resins for covalent immobilization of ligands, and columns for packing affinity resins.

• Antibody Purification Resins and Kits A wide selection of agarose beads, affinity supports and purification kits to purify total or specific antibodies from serum, ascites fluid or culture supernatant; includes many varieties of Protein A, Protein G and Protein A/G resins and kits.  
• Biotin-Binding Affinity Resins and Beads Avidin and streptavidin agarose beads and resins to bind, immobilize and purify biotinylated antibodies and other ligands with high specificity for the avidin-biotin interaction in small- or large-scale affinity purification applications.  
• Fusion Protein Purification Nickel-NTA and cobalt immobilized metal affinity chromatography (IMAC) resins, columns and kits for His-tagged protein purification; Glutathione agarose beads and kits for GST-tagged protein purification.  
• Immunoprecipitation and Pull-Down Kits Several varieties of immunoprecipitation (IP), co-immunoprecipitation (Co-IP) and pull-down assay kits optimized for different sources and types of antibody or other protein affinity systems such as biotinylated and GST-tagged proteins.  
• Magnetic Affinity Separation Particles Paramagnetic particles and activated affinity purification beads for benchtop immunoprecipitation procedures and automated protein separation workflows based on antibody, streptavidin, GST and titanium dioxide ligands.  
• Other Immobilized Affinity Ligands Agarose beads and other resins with specialized immobilized ligands for diverse affinity methods (lectins, metal ions, ribonucleotides, heparin, etc.). Obtain 1000- to 10,000-fold purification of target molecules from crude mixtures.  
• Post-Translational Modification Enrichment Kits Purification resins to isolate and enrich for sample proteins that contain specific post-translational modifications (PTM) including glycosylation (glycoproteins), ubiquitin (poly-ubiquitinated proteins), and phosphorylation (phosphoproteins).  
• Spin Columns and Gravity-flow Columns Different sizes and types of empty purification columns and filter spin columns for packing desalting resins, agarose beads or other affinity supports to use in microcentrifuge or gravity-flow chromatography procedures. 
•  Affinity Resins for Protein Immobilization Agarose beads and other porous resins activated for chemical attachment of affinity ligands (antibodies, glycoproteins, fusion proteins, peptides) to create affinity supports for purification based on specific binding with the immobilized molecule.

Dialysis, Desalting and Buffer Exchange Concentrators, dialysis cassettes, desalting columns and other buffer-exchange devices for removing contaminants and concentrating samples to ready them for long-term storage or to make them compatible with downstream applications.  

• Buffers for Protein Methods Ready-to-use, pre-formulated buffers for protein methods; includes PBS, PBS-T, TBS, TBS-T (Tween-20), Tris-glycine running buffers and transfer buffers, phosphate buffers for protein labeling, and carbonate-bicarbonate buffer for plate coating.  
• Concentrators for Ultrafiltration Centrifuge tube microfiltration devices for sample concentration and buffer exchange of dilute protein samples by centrifugal ultrafiltration through filter membranes; 9K, 20K and 150K MW cutoff varieties available in 7mL and 20mL capacity units.  
• Desalting Columns High-performance centrifuge desalting columns, microplates and cartridges, as well as traditional gravity-flow columns containing gel-filtration resins for efficient buffer-exchange of protein samples by size-exclusion chromatography. Dialysis Tubing, Cassettes and Devices High-performance dialysis membrane products, including Slide-A-Lyzer Cassettes, SnakeSkin Tubing, andother dialysis systems to accommodate nearly any sample volume and molecular weight, or to perform equilibrium dialysis.  
• Ion Exchange Columns Microcentrifuge (0.5mL) and centrifuge (20mL) high-capacity and filter-based ion exchange columns fornegative (anionic) and positive (cationic) separation and purification of proteins or other biological molecules based on charge differences.

Protein Assays and Quantitation Assay reagents, kits and standards to determine total protein concentration of samples containing different buffer components; protein quantitation is often necessary for isolation, separation, and analysis of proteins by biochemical methods.

• Total Protein Assay Kits Bradford assay reagents, BCA assay kits, albumin (BSA) standards and other spectrophotometer and microplate protein assay methods to accurately quantify, determine and measure total protein concentration following cell lysis, labeling or purification.
• Class-Specific Protein Assay Kits Specialized protein assay reagents and kits to estimate and measure specific post-translational modification (PTM) or functional (enzymatic) classes of proteins in biological samples, including glycoproteins, phosphoproteins and proteases.
• Target-Specific Protein Assay Kits Microplate and pull-down kits designed to capture, detect and measure specific proteins and biological sample components by a variety of probe methods (e.g., antibody, dye, substrate, receptor).

Protein Electrophoresis and Staining Precast gels, running buffers, molecular weight markers, and gel stains necessary for sample preparation, loading and analysis when studying proteins by electrophoresis (SDS-PAGE).

• Electrophoresis Sample Prep Kits Clean-up kits and reagents specifically designed to remove contaminants, detergents and other incompatible components from protein samples to make them ready for gel loading and protein electrophoresis.
• Gel-Casting and Polymerization Reagents Polyacrylamide solutions, buffers, polymerizing agents and catalysts for pouring and casting protein electrophoresis gels for SDS-PAGE.
• Loading Buffers for SDS-PAGE Protein sample loading buffers for electrophoresis by reducing or non-reducing denaturing SDS-PAGE in standard Tris-glycine gels and other varieties of mini gels and pre-cast gels.
• Protein Electrophoresis Gels Pre-cast uniform and gradient gels as polyacrylamide mini gels (precast gels) for SDS-PAGE protein electrophoresis, including cassettes and sample-well varieties that provide long shelf-life, fast run-time, and excellent resolution.
• Protein Molecular Weight Markers Unstained, pre-stained and labeled molecular weight markers (MW markers) and protein ladders for SDS-PAGE to facilitate assessment of relative protein sizes in colorimetric and chemiluminescent methods.
• Stains for Protein Gels (PAGE) Coomassie blue, monochromatic silver, fluorescent, infrared and other sensitive or reversible gel stain reagents and kits for total or class-specific detection of proteins in polyacrylamide gels or transfer membranes.

Western Blotting, ELISA and Cell Imaging Blocking buffers, secondary antibody conjugates, and substrate detection reagents (colorimetric, chemiluminescent and fluorescent) for sensitive and consistent protein analysis by Western blotting, ELISA and immunohistochemistry (IHC).

• Secondary Antibodies and Conjugates Polyclonal antibodies for many species of IgG and other immunoglobulins, offered in purified (unconjugated) forms and as conjugates with HRP, AP, biotin and popular fluorophores.
• Biotin-Binding Proteins and Conjugates Streptavidin, NeutrAvidin and avidin protein conjugates for biotinylated antibody probe detection in ELISA and Western blot protocols; includes peroxidase and poly-HRP conjugates and high-intensity fluorescent and infrared conjugates.
• Antibody-Binding Proteins and Conjugates Protein A, Protein G, Protein A/G, Protein L and other antibody-binding proteins available in purified form and as biotinylated or peroxidase-labeled (HRP) conjugates for secondary detection in microplate and membrane assay techniques.
• Blocking Buffers for Protein Methods Traditional protein blocking agents (BSA, casein and milk) and exclusive Pierce blocking buffers (SuperBlock, StartingBlock, Protein-Free) for Western blotting, ELISA and other immunoassay detection methods with microplates and membranes.
• Western Blot Reagents, Kits and Equipment Colorimetric, fluorescent and chemiluminescent Western blot substrates (Pierce ECL, SuperSignal); nitrocellulose and PVDF membranes; X-ray film and accessories; and antibody stripping buffers essential for immunoblotting detection procedures.
• ELISA Reagents, Microplates and Accessories Substrates, buffers, plates and various other supplies to develop and complete ELISA and EIA experiments; includes high-binding capacity coated microplates and colorimetric (TMB), chemiluminescent and fluorogenic enzyme substrates for HRP conjugates.
• Immunohistochemistry Reagents Tissue crosslinkers, cell fixatives, formaldehyde, permeabilization reagents, precipitating enzyme substrates, dyes and staining kits for immunohistochemistry (IHC) and other histochemical detection procedures.
• Nucleic Acid Labeling and Detection Products for labeling, hybridization, electrophoresis and transfer of nucleic acids for Southern blotting, Northern blotting, gel-shift assays (EMSA) and gene expression studies.
• Nucleic Acid Labeling and Detection Products for labeling, hybridization, electrophoresis and transfer of nucleic acids for Southern blotting, Northern blotting, gel-shift assays (EMSA) and gene expression studies.

Protein Interactions Affinity, crosslinking and detection reagents and kits designed to identify and characterize protein interactions (both stable and transient) by immunoprecipitation, pull-down, far-Western blotting, crosslinking and label transfer.

• ChIP Assay Kits and Products Complete kits and supporting reagents for chromatin immunoprecipitation experiments (also called chromatin IP assays or ChIP assays) designed to purify, identify and assess protein-DNA interactions for downstream quantitative PCR.
• Co-Immunoprecipitation & Pull-Down Assay Products Convenient kits to affinity purify protein interaction binding complexes via co-immunoprecipitation (co-IP) or non-antibody affinity system (Pull-Down).
• Label Transfer Reagents Biotin-labeled or iodinatable heterobifunctional, cleavable crosslinkers that can be used to tag a bait protein and then transfer the tag onto an interacting protein following a binding interaction.
• Label Transfer Reagents Biotin-labeled or iodinatable heterobifunctional, cleavable crosslinkers that can be used to tag a bait protein and then transfer the tag onto an interacting protein following a binding interaction.
• Photoreactive Amino Acid Products Photo-Leucine and Photo-Methionine are amino acid analogs that can be incorporated into proteins in actively growing cells. In vivo protein interactions are covalently crosslinked upon UV-light activation of the diazirine moiety.
• Active GTPase Pull-Down Kits and Products Highly efficient in the pull-down of active small Ras, Cdc42, Rac1, Rho and Rap1 GTPases from multiple samples.

Protein Mass Spectrometry and HPLC Reagents and kits for peptide and protein analysis (proteomics) by mass spectrometry (MS) and high-pressure liquid chromatography (HPLC). Includes sample prep tools, mass-tagging (TMT) and isotope labeling (SILAC) kits, and derivatization reagents.

• Mass Tagging Systems for Proteomics Chemical labeling technologies for protein identification and mass analysis by liquid chromatography and mass spectrometry (LC-MS); includes stable isotope labeling reagents, tandem mass tags, and deuterated crosslinkers.
• Protein Sample Prep Tools for Mass Spectrometry Affinity columns, filter plates, and precipitation and contaminant removal kits to enrich, purify, clean up, and recover proteins for downstream proteomics analysis by mass spectrometry.
• Peptide Sample Prep Tools for Mass Spectrometry Affinity columns, resin tips and other kits to fractionate, isolate, purify, clean up, and recover total or post-translational modification (PTM) classes of processed peptide fragments for proteomics analysis by mass spectrometry.
• Reagents for Mass Spectrometry Positive and negative ionization calibration standards and matrices for matrix-assisted laser desorption ionization (MALDI), electrospray ionization (ESI) and other forms of proteomics analysis with mass spectrometers.
• Amino Acid Analysis Reagents and Accessories Colorimetric and fluorescent reagents to accomplish pre-column and post-column derivatization of peptides and amino acids for analysis by HPLC detectors; includes vacuum hydrolysis tubes and quantitation standards.
• Solvents for HPLC Analysis Spectral-grade solvents with low UV-absorption and low peak interference for liquid chromatography (HPLC) and mass spectrometry (MS); includes trifluoroacetic acid (TFA), formic acid and heptafluorobutyric acid (HFBA).
• Laboratory Cleaning Detergents Residue-free detergents for cleaning glassware, utensils, labware and other laboratory surfaces; mild surfactant and phosphate-free formulations contain no harsh acids, do not etch glass and rinse to leave no residue.

Protein and Antibody Labeling Labeling reagents and kits to label an antibody or other protein to make a fluorescent probe, enzyme conjugate, biotinylated binding reagent or iodine labeled tracer for detecting proteins in tissues, cells, or biochemical assays.

• Biotin Labeling Reagents for Proteins Biotinylation reagents and kits to chemically tag and label antibodies, proteins and peptides with biotin at particular amino acid functional groups to create labeled antibody or other probes for streptavidin affinity binding, purification and detection.
• Enzyme Labeling Reagents for Proteins Horseradish peroxidase (HRP), alkaline phosphatase (AP) and other substrate enzymes in purified, activated and kit formats for easy conjugation to proteins and preparation of antibody conjugates for immunoassay procedures.
• Fluorescent Labeling Reagents for Proteins Convenient kits and activated fluorescent dyes for labeling amines, sulfhydryls and other antibody or protein functional groups with FITC, fluorescein, rhodamine or one of several DyLight Fluors to create molecular probes for fluorescence imaging.
• Iodine Labeling Reagents for Proteins Tyrosine addition compounds and tyrosyl activation vessels that replace chloramine T oxidation reagents to efficiently label proteins and other molecules with radioactive iodine (usually 125I), a process called iodination, for use as tracers and probes.
• Metabolic Labeling Reagents for Proteins Bioorthogonal azide-modified amino acids, sugars and other compounds for metabolic incorporation into proteins and macromolecules to enable labeling and chemoselective conjugate with alkyne or phosphine reagents via Staudinger ligation chemistry.

Crosslinking and Protein Modification Reagents to modify proteins by crosslinking, fragmenting, denaturing, reducing disulfides, or attaching various prosthetic groups (e.g. PEGylation) to allow manipulation and study of protein function and interactions in any environment.

• Crosslinking Reagents Chemical crosslinkers and bioconjugation reagents for covalent protein crosslinking techniques to conjugate antibodies, immobilize ligands, attach haptens to carrier proteins, and stabilize folded protein structures and protein interaction complexes.
• Modification Reagents for Amino Acids Chemical agents to modify amino acid side chains on proteins and peptides in order to alter native charges, block or expose reactive binding sites, inactivate functions, or change functional groups to create targets for crosslinking and labeling.
• PEGylation Reagents Activated linear and branched derivatives of polyethylene glycol (PEG) for pegylation and PEG-modification of peptides and proteins via primary amines and sulfhydryl groups to increase solubility, prolong stability and reduce immunogenicity.
• Proteases and Protein-Cleaving Reagents Purified and agarose-immobilized proteases for enzymatic proteolysis (cleavage or digestion) of proteins to facilitate amino acid sequencing, peptide analysis and polypeptide structural characterization.
• Protein Denaturants and Chaotropes Chaotropic and denaturing chemical agents, including urea and guanidine hydrochloride, to disrupt water interactions and promote hydrophobic protein and peptide solubilization, elution, refolding and structural analysis.
• Reducing Agents for Protein Disulfides Purified powders, convenient solutions and solid-phase resins of disulfide reducing agents, including DTT, BME and TCEP, for stabilization of free sulfhydryls (cysteines) and reduction of disulfide bonds in peptides and proteins.

Antibody Production and Purification Carrier proteins, immunogen preparation reagents, isotyping kits, antibody purification resins and immunoglobulin fragmentation kits to easily create and purify antibodies for any immunodetection technique.

• Antibody Production KLH and other carrier proteins to conjugate peptides and other haptens through sulfhydryl, amine or carboxyl groups to elicit production of specific antibodies against almost any molecule. Also includes adjuvants for effective immunization.
• Antibody Isotyping and Characterization Reagent kits for rapid and accurate determination of mouse monoclonal antibody isotype (immunoglobilin class and subclass) and species-specific antibody concentration for screening of hybridoma or ascites antibody production and titer.
• Antibody Purification A wide selection of agarose beads, affinity columns and purification kits to purify total or specific antibodies from serum, ascites fluid or tissue culture supernatant; includes many varieties of Protein A, Protein G, Protein A/G and Protein L.
• Antibody Fragmentation Kits Optimized reagents and kits based on immobilized papain, pepsin and ficin proteases to selectively cleave IgG and IgM immunoglobulin molecules into Fab, Fab', F(ab')2 and Fc antibody fragments for use in immunodetection and immunoassay procedures.

Antibodies Specific polyclonal and monoclonal antibodies to probe, stain, locate, purify and detect virtually any protein, plus secondary antibodies conjugated to the widest array of fluorescent and enzymatic labels for high sensitivity detection.

• Validated Antibodies for Cellular Proteomics Description and menu of specific links to www.pierce-antibodies.com of primary antibodies that have been validated by siRNA knock-down experiments for specific and accurate Western blot detection.

Peptides Custom peptide synthesis of individual peptides, peptide libraries and heavy peptides for antibody preparation, drug discovery, epitope mapping and quantitative mass spectrometry. Keep checking back, as this new section will continue to grow!

• HeavyPeptide Custom Synthesis Service Thermo Scientific HeavyPeptide Custom Synthesis Service provides isotopically labelled, AQUA-grade peptides for the relative and absolute quantitation of proteins at very low concentrations in complex protein mixtures.
• HeavyPeptide FasTrack Service The Thermo Scientific HeavyPeptide FasTrak Service is a 2-step approach to accelerate targeted assay development and absolute quantitation by selected reaction monitoring (SRM) within a controlled-budget environment.
• PEPotec Immuno Custom Peptide Libraries Thermo Scientific PEPotec Immuno Peptide Libraries are fully synthetic custom libraries that support high throughput screening assays to map epitopes or identify immunogenic sites in proteins.
• PEPotec SRM Custom Peptide Libraries Thermo Scientific PEPotec SRM Peptide Libraries are fully synthetic peptides customized for the development of high-throughput single reaction monitoring (SRM) and multiple reaction monitoring (MRM) assays in quantitative mass spectrometry (MS) workflows.
• Imject KLH-Peptide Conjugation Service Save time and avoid sub-optimal immunogen-peptide conjugation for antibody production by using the Thermo Scientific Imject Mariculture KLH-Peptide Conjugation Service

Target-Specific Assay Kits Sandwich ELISA, EIA and other assay kits for detection and measurement of specific cytokines, chemokines, cell signaling proteins, transcription factors, and other cellular targets.

• ELISA Kits 96-well plate sandwich ELISA kits and matched antibody pairs to assay cytokines, growth factors and soluble receptors in serum, plasma, media and cell culture supernatant samples from human, mouse and other species.
• In-Cell ELISA Kits for Specific Targets Colorimetric and infrared fluorescent assay kits for whole-cell ELISA quantitation of specific intracellular proteins in plates of cultured cells (96-well or 384-well).
• In Vivo Imaging Probes and Reagents Specialized reagents to detect target proteins or reactions in vivo, such as caspase activation indicative of apoptosis using our popular DyLight fluorescent reagents.
• Accessory Reagents for ELISA and ELISPOT Blocking buffers, wash buffers, stop solution, substrates and peroxidase conjugates for cytokine ELISA methods.
• Recombinant Proteins Recombinant cytokines, chemokines and growth factors for bovine, human, mouse, porcine, rat and other species. Fully validated and quality-tested to ensure high biological activity.